Regulatory

Part:BBa_K4156101:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


pLldR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the Llprd operon, we were the first to use a combination of the promoter hyper-spnak with RBS spoVG to initiate downstream sequences. After the lldR regulatory protein with the LldPRD promoter, we also introduced the RiboJ and RBS B0034 sequences to enhance transcriptional efficiency.


Source

Experimental constructed.

References

Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997